In this study. we employed a novel approach to improve the serotonin-responsive ssDNA-wrapped single-walled carbon nanotube (ssDNA-SWCNT) nanosensors. combining directed evolution and machine learning-based prediction. Our iterative optimization process is aimed at the sensitivity and selectivity of ssDNA-SWCNT nanosensors. https://www.iguanasellers.shop/product-category/mechanical-pencil/